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Ivanov A. V., Tessman A. B., Nesterenko P. N., Vakshtein M. S., Matveyev A. V.
Ionic Strength of the
Initial Solution as an Effective Way of Optimization in Chromatofocusing
Abstract
Optimization
of pH gradient shape in chromatofocusing is possible due to the right choice of
ionic strength of a start solution and an eluent. The applicability of simple,
single-component eluents with the combination of high ionic strength of start
solution for the generation of quasi-linear pH gradients in chromatofocusing of
amino acids and proteins was demonstrated in the first time.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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