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Sumbatyan N. V., Topin A. N., Taranenko M. V., Korshunova G. A.
Synthesis of
Novel Photoactive Enkephalin-Like Peptides
Abstract
Two novel
photoaffinity analogues of enkephalin containing
p-[3-(trifluoromethyl)-3H-diazirin-3-yl]-L-phenylalanine or
p-azido-L-phenylalanine residues in position 4 ([D-Ala2, Phe(Tmd)4,
D-Leu5]-enk, I, and [D-Ala2, Phe(N3)4, D-Leu5]-enk, II) were synthesized by
classic technique in solution. Photochemical properties of the analogue I and
II were studied under UV irradiation
at 350 nm. Optimal time of photolysis for ligand-receptor complex was determined. Binding properties of the analogues with opiate
receptors were investigated. In the [H3]DAGO and [H3]DSLET binding assays the
analogue I showed almost the same affinity for m-receptor and the
lower affinity for d-sites compared to the parent DADLE, while the
analogue II retained affinity and preference for m-receptor.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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