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Ageeva E. V., Gladilin A. K., Levashov A. V.
Stabilization of antibodies by complex
formation with heparine in water-ethanol mixture
Abstract
Complex formation with polyelectrolyte has been
suggested as an approach for antibodies stabilization against inactivation in
water-organic media. It is established, that complex formation with
polyelectrolyte leads the broadening of the range of organic solvent
concentration where antibody retained their immunoreactivity. It creates the
prerequisites for immunochemical assay carrying out in the media with high
content of organic component. It has been found, that the main
physical-chemical reason of antibodies stabilization in water-organic mixtures
is maintenance of their native conformation as a result of multipoint interaction
with polyelectrolyte.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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