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Sukovataya I. E., Tyulkova N. A
Effects of organic solvents on bacterial
bioluminescent reaction.
Abstract
The activating and inactivating effects of
methanol, ethanol and acetone on the activity of luciferases from two types of
luminous bacteria Photobacterium
leiognathi and Vibrio harveyi was analyzed with kinetic graphic methods. The
long-chain aliphatic aldehydes, e.g. decanal (C10), dodecanal (C12),
tetradecanal (C14) were used as substrates of a different
hydrophobicity. The kinetic analysis of inhibition and activation types indicated
that the molecules of organic solvents could be bound to the active center of
each of luciferases.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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