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Faenkova V. P., Subbotina T. F., Galebskaya L. V.
The degradation of streptokinase by renal
cathepsin B
Abstract
Cathepsin B was isolated from human kidneys and
incubated at 37oC and pH 5.0 with streptokinase of the preparation
Streptasa ("Belmedpreparat", Minsk). Cathepsin activity against
streptokinase was studied with gel-filtration, PAAG-electrophoresis and with
functional tests. The appearance of degradation products (8-10 kDa and about 50
kDa) was essential after 5 min of incubation but didn't manifest in any change
of the preparation plasminogen-activating properties. Further streptokinase
degradation caused first elongation of plasminogen activation and then
inhibition of fibrinolysis up to its complete abrogation.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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