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A. S. Romanenkov, O. A. Borisova, E. A. Kubareva, T. S. Oretskaya, V. G. Metelev
Synthesis
and properties of oligodesoxyribonucleotides containing phosphoryldisulfide
moiety in the predetermined position of carbohydrate-phosphate backbone
Abstract
The synthesis of oligodeoxyribonucleotides with
internucleotide phosphoryldithio group was optimized. The lowering of
incubation mixture pH to 4.5 - 5.0 allowed to lessen substantively the reaction
time and to increase the yield of target compounds. It was demonstrated that
modified oligodeoxyribonucleotides are able to interact easily with mercapto
groups of dithiotreitol and cysteine via thiol-disulfide exchange, but
they are stable to lysine treatment.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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