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M. S. Vakstein, A. V. Ivanov, P. N. Nesterenko, E. M. Khasanova
Application
of Carbonic Acids for the Descending pH Gradient Formation in the Column Filled
with Hyper Cross-Linked Polysterene with Attached Carboxilic Groups
Abstract
For
descending pH gradient formation in the cation-exchange column, filled with
hyper cross-linked polysterene with attached carboxilic groups, the
chromatofocusing technique has been applied. The column was presaturated by
start buffer solution (10 mM Tris-HCl). The possibility of pH gradients
formation by using of single-component eluents, based on carbonic acids, has
been shown. The addition of NaCl to the start buffer and in the eluent allows
to form quasi-linear pH gradient in range
7,5– 3,5.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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