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M. S. Vakstein, A. V. Ivanov, P. N. Nesterenko, E. M. Khasanova

Application of Carbonic Acids for the Descending pH Gradient Formation in the Column Filled with Hyper Cross-Linked Polysterene with Attached Carboxilic Groups

Abstract

For descending pH gradient formation in the cation-exchange column, filled with hyper cross-linked polysterene with attached carboxilic groups, the chromatofocusing technique has been applied. The column was presaturated by start buffer solution (10 mM Tris-HCl). The possibility of pH gradients formation by using of single-component eluents, based on carbonic acids, has been shown. The addition of NaCl to the start buffer and in the eluent allows to form quasi-linear pH gradient in range 7,5– 3,5.
Moscow University Chemistry Bulletin.
2003, Vol. 44, No. 4, P. 258
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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