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K. V. Stepanov, A. V. Pirogov, M. A. Dikunetz, O. A. Shpigun
A technique of synthesis of amino
acid phenylthiohydantoines for the quantitative protein compositional analysis
using capillary electrophoresis
Abstract
A technique for the derivatization of amino-acids
with phenylisothiocyanate has been developed for the capillary electrophoretic
applications. The derivatization technique based on the reaction between of
dried amino-acid’s hydrolysate and phenylisothiocyanate with the following
cyclization of phenylthiocarbamates in an aqueous solution of a triflouracetic acid(a scheme is presented). An
additional procedure of the sample and the derivatives purification for
capillary electrophoresis is described. The by-products the of derivatization
and purification procedures were separated, determined and compared by HPLC.
The technique has been tested on samples of a protein hydrolyzate and a fish
flour.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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