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A. J. Casas-Reyes, F. Cerda-Ramirez, Y. Garza-Garcia, N. Balagurusamy, A. D. Ilyina
Application of bioluminescence
method for quality control of culture media and bacteria applied to standard
norm (NMX-AA-042-1987)
Abstract
The aim of the study was to
develop a bioluminescence assay for the rapid monitoring of the quality of the
bacterial culture medium and quality of standard bacterial strain by way of ATP
detection, as well as to define the relationship between ATP and the bacterial
population estimated by MPN and CFU methods. The growth kinetics of E. coli in the Brilliant green bile
and Lactose broth, suggested by the NOM (Mexican Official Norms) were studied.
The influence of pH, ionic force, and temperature was also evaluated for E.
coli and E. coli co-inoculated with Pseudomonas spp. ATP was
detected by the bioluminescent method after dimethyl sulfoxide treatment and
simultaneously the bacteria present in the samples were enumerated by plating
and MPN techniques. All the parameters tested to compare the growth of bacteria
in brilliant green bile and lactose broth, were statistically significant. A
strong inhibition of growth was observed in both media containing 5% NaCl. An increase in specific growth constant of E.
coli was noted in presence of Pseudomonas spp. The relationship
between ATP, CFU and MPN of the cultures was established and it was observed
that the ATP assay has good correlation with CFU and MPN techniques.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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