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A. D. Ilyinб, E. P. Segura-Ceniceros, M. G. Hernбndez-Vazquez, R. Chбvez-Chбvez, Ye. Bogachiova, L. E. Elizalde-Herrera
Improvement of PGH-synthase stability in pectin
films used in healing of skin
wounds
Abstract
Prostaglandin H synthase (PGHS, E.C. 1.14.99.1) and
prostaglandins mediate a variety of physiological and pathological processes.
Herein, we report a new method of PGHS immobilization applicable to the
treatment of skin injuries, using natural polymer pectin as a support matrix.
PGHS was isolated from bovine vesicular glands, immobilized on pectin, and
tested for stability and activity. The
enzyme retained up to 88% of its activity at –15°C during first 5 days and 41%
after 60 days of storage. The effectiveness of immobilized PGHS on tissue
repair was tested in CD1 mice by measuring linear size of PGHS treated and
untreated wound. Application of PGHS accelerated wound healing and stimulated
prostaglandin E2 (PGE2)
generation in the wound. Immobilized PGHS also increased specific rate of
healing progression (m*) that was calculated as the tangent of the inclination
angle of lines obtained in semi-logarithmic coordinates. A kinetic scheme was
proposed to describe the process of wound healing as the function of PGHS
concentration.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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