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A. S. Krapivin, Zh. V. Samsonova, N. A. Uskova, N. L. Ivanova, A. M. Yegorov
Solid-Phase
Immunoferment Analysis of Bisphenol A: Influence of Immunoreagent Structures on
Sensitivity and Specificity of Analysis
Abstract
A
range of ELISAs in indirect and direct formats for the determination of
bisphenol A was developed. Bisphenol A carboxymethyl ether, bisphenol A
carboxypropyl ether and 4,4-bis(4-hydroxyphenyl)valeric acid were coupled with
bovine serum albumin (immunogens for the production of polyclonal antibodies),
ovalbumin and horseradish peroxidase. Analytical parameters of heterologous and
homologous combinations of immunoreagents were compared. In general the
indirect assay was more sensitive and specific than the direct one.
Additionally, in the indirect assay using of heterologous combinations of
immunoreagents allowed to increase assay sensitivity and specificity. The highest
sensitivity was obtained for the antibodies produced against a conjugate of
bisphenol A carboxypropyl ether with bovine serum albumin and a conjugate of
4,4-bis(4-hydroxyphenyl)valeric acid with ovalbumin. The detection limit of
bisphenol A in phosphate buffer was 0.03 ng ml–1. This combination of
immunoreagents also demonstrated the highest specificity to bisphenol A since
the antibodies cross-reacted only with 4-cumilphenol (11%), bisphenol E (5%)
and bisphenol S (2 %).
Copyright (C) Chemistry Dept., Moscow State University, 2002
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