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N. A. Moroz, D. Ya. Gursky, N. N. Ugarova
Stabilization of ATP-reagents containing firefly luciferase
Luciola Mingrelica by polyols
Abstract
ATP-reagents containing 2–20 % of ethylene glycol,
glycerol, or PEG-8000 were prepared. The 20–40 % decrease of the ATP-reagent’s
activity in the presence of polyols was demonstrated, it was prevented by adding of 0.75 mM Triton X-100. Concentration
of polyols 10 % was found to be optimal. Kinetics of thermoinactivation of
ATP-reagents in the presence of polyols at 37, 20 and 4єC was investigated. At all temperatures, the stability of ATP-reagents in the presence of
polyols was increased 2–5 times in comparison with that of standard
ATP-reagent. The solutions of the stabilized ATP-reagents may be stored at 4 єC for 180 h without loss of activity. The
ATP-reagents activity half-life under these conditions was higher then two
weeks.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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