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K. V. Goncharenko, S. S. Savin, V. I. Tishkov
Membrane
detection of nanogramm amounts of formate dehydrogenase
Abstract
The method for detection of nanogramm amounts of formate dehydrogenase
on membranes was developed. The optimization of the method was carried out for
determination of the best mediator and concentration and tetranitrotetrazolium
blue. It is found that 5-methyl-phenazine methosulfate is more effective
mediator than phenazine methosulfate. Different types of membranes were tested
and it was shown that nitrocellulose membrane provides the lowest background
and the highest sensitivy. Under optimal conditions the detection limit was 15
ng of the enzyme in probe.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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