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S. V. Uglanova, M. V. Popov, V. S. Kurova, E. V. Batrakova, D. Manickam, A. V. Kabanov, N. L. Klyachko
Stabilization
of enzymes-antioxidants by complex- and conjugate formation with
block-copolymers: prospects to cns treatment
Abstract
Nano-sized particles with radius of 16±2 nm based on complexes and
conjugates of highly active superoxide dismutase and catalase with
polyelectrolyte block copolymer poly(ethyleneimine)-poly(ethylene glycol) and
similar bienzyme systems were obtained. Mass-spectrometry was used to confirm
cross-linking of enzyme and block copolymer molecules in nanoparticles formed.
Significant increase of SOD and catalase stability (up to 4 times) towards
proteolytic degradation under chymotrypsin and trypsin action for 3 h at 37C was revealed when enzyme-containing nanoparticles were used for experiment. Antioxidant
enzymes-containing polyelectrolyte nanoparticles are seemed to be prospect for
BBB penetration and CNS drug delivery.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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