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V. A. Kudrinskaya, S. G. Dmitrienko, Yu. A. Zolotov
Spectrophotometric
determination of flavonoids by the reaction with (4-nitrophenil)diazonium
tetrafluoroborate
Abstract
(4-Nitrophenil)diazonium
tetrafluoroborate (4-NPD) has been suggested as a reagent for the
spectrophotometric determination of flavonoids. It has been shown that in
alkaline medium 4-NPD enters into the diazotization reaction with quercetin,
naringenin, chrysin, morin, rutin and naringin to form peach-coloured products.
The conditions for the spectrophotometric reaction have been optimized.
Spectrophotometric method for the determination of flavonoids with detection
limits (1.5 โ3.9)ยท10โ7M (0.05 โ 0.16 mg/mL)
has been developed. Quercetin has been determined in some pharmaceutical
compositions.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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