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A. A. Bendryshev, E. B. Pashkova, A. V. Pirogov, O. A. Shpigun
Determination
of water soluble vitamins in vitamin premixes, biologically active dietary
supplements and pharmaceutical preparation by HPLC with gradient elution
Abstract
Influence of the pH of
the mobile phase and temperature on the chromatographic separation of 14
vitamins and vitameric forms on four different stationary phases was
investigated. The chromatographic parameters for simultaneous determination of
12 vitamins and vitameric forms were suggested. The best separation was
achieved under the following conditions: gradient elution mode, pH of eluent
A(0,6% phosphoric acid) 1,5–1,8; eluent B – acetonitrile. In order to separate
nicotinic and ascorbic acid, stationary phases Luna C18(2) and Gemini C18, and
to separate riboflavin and riboflavin-5¢-
phosphate correspondently – stationary phases Synergi Hydro RP and Zorbax
SB-C18 are more preferable. The suggested technique was applied for the
determination of vitamins in commercial samples of multivitamin pharmaceuticals
and premixes. The obtained results were in good conclusion with passport data.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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