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M. I. Koksharov, D. V. Smirnova, S. GAbbasova, N. N. Ugarova

Fusion protein of luciferase Luciola mingrelica–biotin carboxyl carrier protein: production, properties, application


A plasmid was constructed by genetic engeneering methods that codes a fusion protein (4TS-bccp87) including the thermostable mutant of Luciola mingrelica firefly luciferase (4TS) and the carboxyl-terminal 87 residues of E. coli biotin carrier protein (bccp87).It was shown that about 60% of the fusion protein was biotinilated after the expression in E. coli BL21(DE3). Catalytic properties, thermostability and bioluminescence spectra of the fusion protein were shown to be similar to that of the initial luciferase. It was demonstrated that streptavidin-biotinylated luciferase complex can be used in ELISA for the assay of Salmonella typhimurium cells in the interval from 104 to 5·106 CFU/ml.
Moscow University Chemistry Bulletin.
2011, Vol. 52, No. 4, P. 291

Copyright (C) Chemistry Dept., Moscow State University, 2002
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