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N. V. Borzenkova, A. V. Kislaya, I. A. Veselova, T. N. Shekhovtsova
Application of heme-containing biocatalysts
for the oxidation and determination of sulfur-containing organic compounds
Abstract
The conditions of the oxidation of
dibenzothiophene and aliphatic phenothiazines (promazine and chlorpromazine) in
the presence of heme-containing biocatalysts (such as horseradish peroxidase
and hemoglobin) were optimized. It was shown that hemoglobin is an effective
catalyst for fast and selective conversion of dibenzothiophene to S-oxide
(yield 91±2% for 5 min); the using of horseradish peroxidase is a promising
catalyst for the phenothiazines oxidation. The influence of the polar organic
solvent dimethyl sulfoxide on the kinetic parameters of promazine oxidation in
the presence of heme-containing biocatalysts was studied. The procedures for
the phenothiazines determination based on the spectrophotometric measurement of
the oxidation reaction rate were developed. The analytical concentration ranges
in aqueous medium for the determination of promazine and chlorpromazine in the
presence of peroxidase were 10–100 and 10–200 M, respectively, and 25–500 M for the
determination of promazine in the presence of 10% (v/v) dimethyl sulfoxide.
Key words: phenothiazines,
dibenzothiophene, selective oxidation, determination, biocatalysts, organic
solvents.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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