N. V. Borzenkova, A. V. Kislaya, I. A. Veselova, T. N. Shekhovtsova Application of heme-containing biocatalysts for the oxidation and determination of sulfur-containing organic compounds Abstract The conditions of the oxidation of dibenzothiophene and aliphatic phenothiazines (promazine and chlorpromazine) in the presence of heme-containing biocatalysts (such as horseradish peroxidase and hemoglobin) were optimized. It was shown that hemoglobin is an effective catalyst for fast and selective conversion of dibenzothiophene to S-oxide (yield 91±2% for 5 min); the using of horseradish peroxidase is a promising catalyst for the phenothiazines oxidation. The influence of the polar organic solvent dimethyl sulfoxide on the kinetic parameters of promazine oxidation in the presence of heme-containing biocatalysts was studied. The procedures for the phenothiazines determination based on the spectrophotometric measurement of the oxidation reaction rate were developed. The analytical concentration ranges in aqueous medium for the determination of promazine and chlorpromazine in the presence of peroxidase were 10–100 and 10–200 M, respectively, and 25–500 M for the determination of promazine in the presence of 10% (v/v) dimethyl sulfoxide.Key words: phenothiazines, dibenzothiophene, selective oxidation, determination, biocatalysts, organic solvents.
Copyright (C) Chemistry Dept., Moscow State University, 2002 |
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