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A. N. Malyavko, O. A. Petrova, M. I. Zvereva, O. A. Dontsova

Development of a system to test for the presence of any nontelomeric nucleotide on the 3-chromosomal end

Abstract

Amount of telomeric repeats in the telomeres correlated with proliferative potential eukaryotic cells. The lengthening of telomeres and telomerase work is regulated by numerous ways. One of this way is based on the elongation of the 3′-end of chromosome for additional nucleotide that is not part of the sequence of the telomeric repeat. This work presents an approach for testing the presence of an additional nontelomeric nucleotide at the 3′-end of the G-strain of chromosomes. The applicability of this method on the example of thermotolerant yeast H. polymorpha was shown. The possibility of modifications of the method to test additional nucleotides at the 3-end of the chromosomes of other organisms is discussed.
Key words: telomerase, telomeres, H. polymorpha, test of terminal nucleotide on 3′-end of a chromosome, the regulation of telomere length.
Moscow University Chemistry Bulletin.
2016, Vol. 57, No. 1, P. 31
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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