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S. Yu. Zaitsev
Dynamic surface tension parameters of the
protein–lipid–solt solutions as a system
modeling of blood plasma
Abstract
Measurements of the dynamic surface tension
(DST) of liquids is one of the simplest and fastest methods for the analysis of
various chemical solutions and biological liquids. Solutions of the ternary
systems based on bovine serum albumin (BSA), natural phosphatidylcholine (PC)
and sodium chloride were investigated. It is found that at short times (0.1–1.0
s) the major impact had salt content (especially at high concentrations), but
in the medium and long times (over 1.0 second) – BSA concentration, whereas the
changes in the PC concentration (in the range usual for blood) had little
impact on most of the DST parameters. The most informative (Among the seven
studied DST parameters) was the changes (at 22–68%) of tilt tensiogram values λ0, λ1 and λ2. This allowed the determination of the optimal concentrations
ranges: BSA (60–80 g/l), PC (1–4 mM), NaCl (130–150 mM) in the mixture, which
corresponded to their content in blood plasma of humans and animals. For four
parameters σ1 ... σ4
found only small correlations with the changes in the ratio of three components
in the mixture. The resulting patterns are important for complement basic
database and for predictive modeling in biology and medicine.
Key words: solutions, bovine serum albumin,
phosphatidylcholine, dynamic surface tension, blood plasma.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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