A.A. Dzyabchenko, M.A. Proskurnin, A.G. Abroskin and D.V. Chashchikhin
J. Chromatogr. A., v. 827, p. 13-20 (1998)
ABSTRACT. A dual-beam thermal lens spectrometer is used as a detector in HPLC. An algorithm for chromatogram handling, which is based on fast Fourier transform, is developed. The algorithm allows a considerable increase (1.5 to 2 orders) in the sensitivity of TLS-HPLC determinations comparing to HPLC with conventional spectrophotometric detection. A comparison of the methods for calculating the performance characteristics from peak heights (conventional approach) and peak areas showed that the latter variant gives more reliable results. The experimental set-up and developed methods were used for determination of Co and Ni in the form of their pre-synthesised chelates with 4-(2-pyridylazo)resorcinol; the limits of detection are 3x10-8 and 1x10-7 M, respectively.