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Andrei Yu. Laptev, Nina B. Rozhmanova, Pavel N. Nesterenko
Determination of sucralose in combined sweeteners on a complexing
sorbent under conditions of hydrophilic interaction liquid chromatography
Abstract
Adstract. The article is devoted to the
development of a simple chromatographic analysis for the determination
artificial sweetener sucralose at low concentrations in combined sweeteners.
The method of hydrophilic interaction liquid chromatography (HILIC) using a
silica with immobilized 2-hydroxyethyliminodiacetic acid (HEIDA) groups in La3+
form as a stationay phase is developed to determine low concentrations of
sucralose in the presence of large excess of erythritol, using a relative
preconcentration due to different solubility in acetonitrile. The best
separation of sucralose and erythritol was achieved using a acetonitrile:water
(95:5 vol.%) mixture as the mobile phase. The refractive index detector was
used for quantitative determination of sucralose in combined sweeteners with
limit of detection (LOD) of 28 μg/ml.
Key words: hydrophilic interaction liquid
chromatography, chelating adsorbent, 2-hydroxyethyliminodiacetic acid,
sweeteners, sucralose
Copyright (C) Chemistry Dept., Moscow State University, 2002
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