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Ivanov A. V.
Induced Ascending pH Gradients in Liquid Chromatography with Flowing
Retentive Stationary Phase
Abstract
The possibility of induced increasing pH gradients formation in the
liquid chromatography with flowing retentive stationary phase was shown. The
tephlonic and metallic capillars (internal V = 1 ml) were used as open
chromatographic columns with flowing retentive liquid phase; water-methanolic
or water-acetonitilic borate buffer solutions were used as mobile phase. The
octanoic and heptanoic acids or their mixture (1:1), inducing the pH gradient
formation within open capillar, were simultaneously the flowing retentive
liquid phase (stationary phase). The more smooth increasing pH gradients from
5,6-5,8 to 9,1 were induced in system: 5 mcl of octanoic acid, mobile phase -
20 mM Na2B4O7 in 30% CH3OH at flow
rate 0,2 - 0,3 ml/min.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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