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Sirotkin V., Mukhametzyanov T., Solomonov B.
UV/Vis-spectrophotometric study of the
interaction of proflavine with a-chymotrypsin
in water-acetonitrile mixtures
Abstract
In present work the binding of proflavine with
bovine pancreatic a-chymotrypsin (CT) in various water-acetonitrile mixtures has been
measured by means of UV/Vis-spectrophotometry at 25oC. Thermodynamic
activity of water in organic solvent was varied from 0.11 to 1.0. The
spectrophotometric results on proflavine binding have been analyzed in
comparison with literature results on corresponding changes in the protein
conformational mobility and FTIR-data on water state in acetonitrile.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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