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T. V. Kuznetsova, B. I. Shevelev, J. S. Keruchenko, A. B. Shevelev
New system for TNF-alpha quantification in clinical samples
Abstract
Full-length TNF-alpha and its truncated derivative with 18
N-terminal amino acids replaced to T7-tag were produced in E. coli using
artificial genes and rendered inclusion bodies. Purification and refolding
methods were established for the recombinants, their biological activity was
demonstrated. Polyclonal antibodies against TNF-alpha were raised.
ELISA-Sandwich test-system for TNF-alpha quantification with 100 pg/ml
sensitivity was engineered. The system was shown to be applicable for detection
of the TNF-alpha elevation in human blood.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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