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D. A. Skvortsov, M. I. Zvereva, M. P. Rubtsova, L. S. Pavlova, A. A. Petrenko, F. L. Kisseljov, O. A. Dontsova
Optimized
detection method of telomerase activity in cancer diagnostic
Abstract
Telomerase is a ribonucleoprotein complex, which copy an internal RNA
template to synthesize telomere DNA, which are shortened during cell divisions.
Telomerase active in about 80-90% of cancers, but not in most of somatic
tissues. We optimized conditions for extraction from small tissue samples (<0,05 g) and selected optimal concentrations of tissue extract concentration for cervical lesions. Two
concentations of extracts in reaction were used to take into account in-sample
inhibitors. We use these conditions for several cancerous and normal samples of
lung and kidney, these conditions are identical for different types of cancer.
There are a lot of investigations of telomerase activity using different types
of TRAP (Telomere Repeat Amplification Protocol). The question of this study is
could we compare results for clinical materials received by TRAP with
radioactive and Sybr green detection. We compare this two types of detection on
the several samples of cervical intraepithelial neoplasias and conclude, that
sensibility is equal.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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