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E. I. Martirosova, I. L. Zhuravleva, I. G. Plashchina, A. S. Petrovskii, N. G. Loiko, G. I. El-Registan
Controlling
of the catalytic activity and functionality of hen egg white lysozyme by
alkylhydroxybenzenes
Abstract
The study demonstrates the ability of alkylhydroxybenzenes (C7-AHB
homolog) to stimulate the enzymatic activity of hen egg white lysozyme and the
efficiency of hydrolysis reactions of non-specific substrates. The influence of
both the C7-AHB concentration and the C7-AHB form
(primary and oxidized), as well as the incubation time of the mixture of
lysozyme and C7-AHB on the activity of lysozyme and thermodynamic
parameters of its denaturation has been studied using DSC. Kinetic parameters
of chitin hydrolysis by C7-AHB -modified lysozyme have been
estimated.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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