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O. A. Minikh, L. Y. Brovko, M. W. Griffiths, N. N. Ugarova
Specific
E. coli B detection
using T4 Bacteriophage, nano-filters and ATP-bioluminescence
Abstract
Lytic bacteriophage and filters based on nano-aluminium fibers “Disruptor
4601” were proposed to detect specific bacteria by ATP-bioluminescence. As
a model system, system of T4 bacteriophage – bacteria E. coli B was
chosen. Modifications of nano-filter based method with wild type T4
bacteriophage allow either quantitative detection of E.coli B with
detection limit as low as 103 CFU/mL or accurate detection E.coli
B at the 60-fold excess of S. typhimurium. Thus, T4 phage coupled
with nano-filter media method performs much better than T4 coupled with the standard
phage-mediated ATP-bioluminescence method. Increase in testing time required is
not essential.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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