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O. V. Sergeeva, D. Ye. Burakovsky, P. V. Sergiev, T. S. Zatsepin, M. Tomkuviene, S. Klimasauskas, O. A. Dontsova
Methyltransferase as the method of fluorescent label
site-specific induction
Abstract
We investigate the possibility of
usage the analog of S-adenosylmethionine - pent-2-en-4-ynyl
S-adenosylhomocysteine (AdoEnYn) as a co-factor for the rRNA methyltransferase.
We select the conditions for the cycloaddition rection of fluorescent label
azide with the analog of SAM. The functional activity of ribosomes E.coli
was tested in different conditions. It was found thatincorporation of the alkynylradicalis successfulanddoesn’t change thefunctional activityof the ribosomes,but nextcycloaddition reaction lead to the inactivationof the ribosomes.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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