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J. G. Novitskaya, A. A. Litvin, V. P. Zherdev, E. V. Blynskaya, S. E. Kondakov
The quantitative analysis of caffeine and
its metabolites in rat blood plasma with HPLC
technique, as a method for determination of metabolic relations
Abstract
The technique of quantitative
determination of caffeine and its metabolites in biological fluids (rat blood
plasma) with use of a high performance liquid chromatography with UV-detecting
was developed. The limits of quantitative detection of caffeine, paraxanthine, theobromine
and theophylline were 10 ng/ml, 1,3,7 – three methyluric acid − 25 ng/ml
respectively.
Key words: caffeine, theobromine,
theophylline, paraksantin, 1,3,7-trimetiluric acid, high performance liquid
chromatography, quantified.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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