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I. A. Nechepurenko, A. V. Dorofeenko, A. P. Vinogradov, E. G. Evtushenko, I. N. Kurochkin
Signal enhancement from fluorescently
labeled exosomes: theoretical analysis of fluorescence in the vicinity of
plasmonic nanoparticle
Abstract
An approach for measurement of exosomes
concentration in biofluids based on non-specific labeling by fluorescent dye
followed by specific fluorescence enhancement by plasmonic particle is
proposed. Theoretical evaluation of proposed scheme is performed. A model of
the interaction of the incident wave, the fluorophore and the plasmonic
nanoparticles is developed. Calculations of the amplification of molecule
fluorescence near a gold or silver nanoparticles are performed with the account
of actual dispersion of the metal dielectric permittivity. Physical mechanisms
of the fluorescence enhancement are described. These are change of the local
field intensity by the nanoparticle, difference in the radiation efficiency of
the fluorophore and the nanoparticles, as well as superradiance.
Key words: exosomes, fluorescence
enhancement, plasmons, metallic nanoparticles.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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