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Tatiana A. Bogush, Anna N. Grishanina, Elena A. Bogush, Alexander M. Scherbakov, Ekaterina A. Ogurechnikova, Anna B. Ravcheeva, Evgeniya M. Kapura-Brekhovskikh, Mikhail Ya. Melnikov
Immunofluorescent assessment of
vimentin de novo expression in serous ovarian
cancer cells as a potential predictor of platinum and taxane drugs efficacy
Abstract
Abstract. De
novo expression of the mesenchymal protein vimentin in cells expressing
epithelial cytokeratins was quantified by double immunofluorescence staining of
serous ovarian cancer samples (n = 56). It was shown
that the median value was 1.6 times higher (p = 0.006) in the group of
resistant compared to sensitive tumors (relapse of the disease after
chemotherapy with platinum and taxanes for ≤ 6 mo. and ≥ 12 mo.).Conclusion.The level of de novo expression of vimentin is a molecular predictor of the
effectiveness of platinum and taxanes with an estimated specificity and
sensitivity of the analysis of more than 80% with a cutoff for sensitive and
resistant tumors of 42.5%.
Key words: immunofluorescence assay, flow
cytometry, serous ovarian cancer, vimentin, predictor, platinum/taxane drugs
Copyright (C) Chemistry Dept., Moscow State University, 2002
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